Abstract: Eco-hydrological impacts caused by urbanization are important issues in protecting and restoring river ecosystem.Given the importance of flow regime in maintaining river ecosystem integrality,an eco-hydrological indicator system including flow duration curve,peak flow frequency exceedance curve and T_0.5 (The fraction of the simulation period that the peak flow rate exceeds the peak flow of the predevelopment 0.5-year storm) were developed by analyzing characteristics of current indicators and their feasibility in data-lacking area.Urbanization development degrees and their eco-hydrological impacts were simulated in a hydrological model SWMM (Storm Water Management Model).Results demonstrated that the higher imperviousness,the greater instantaneous flow,peak flow,frequency,duration and smaller T_0.5.indicating a negative effect of urbanization on the river in ecology.