Abstract: Scientific and reasonable water conveyance control strategy is the basis for ensuring the safe and stable operation of long-distance water diversion projects. According to the control strategy, the goal of water supply plan to each sluice is realized. On the basis of summarizing the research results of the existing water conveyance dispatch control, combining with the actual operation of the dispatch, considering the robustness of target water level control, the constraint of water level drop and the co-ordination of upstream and downstream water regimes, the water conveyance control strategy is improved. The real-time dispatch control strategy based on water flow change and coupling water level-amplitude is put forward. According to the change of flow, the water balance analysis and macro-control are carried out, and the micro-regulation is implemented according to water level and its amplitude. The model is applied to the typical condition of water level adjustment in the channel section of Middle Route Project of South-to-North Water Diversion in China. The water level in front of the control sluice of Fenzhuang River is successfully reduced by 0.5 m according to the requirements through the long water conveyance dispatch of about 1 100 km between the channel headwork and the test section, and other channel sections keep running smoothly. Owing to that the traditional control strategy does not consider the upstream and downstream water regimes and water level drop limit, the water level in front of the control sluice of Fenzhuang River suddenly decreases, and its downstream water level greatly increases during the tests. The validity of the proposed model is verified through case studies.