Abstract: The interruption of human with the ecosystem of a river basin will change the land use dramatically such as reducing the seasonal flood area,losing the natural wetland,decreasing the diversity of habitat and biology and so on.Based on the holistic theory of river basin,the biodiversity theory,the patience theory and the landscape ecology theory,the influences of various land uses on ecological water requirement are analyzed.The results prove that the ripatian natural plant ecosystem can allocate the water quantity,improve the water quality of river basin,lower the risk of a river basin threatened by the shortage of water resources, and ensure the ecological water requirement on longer time and wider space scales.So the reasonable measures about land uses including protecting wetland,ripatian forest and island water ecotone should be taken in order to make the ecosystem sustainable development of a river basin.