Abstract: Aiming at the characteristics of complexity, dynamism and uncertainty among water transfer, water diversion and water supply in joint regulation of large-scale inter-basin water supply reservoir group, on the basis of bi-level programming model, applying game theory, tri-level programming model of inter-basin reservoir group water supply operation rules is built up and inter-basin reservoir group optimization operation rules after combining water transfer, water diversion and water supply is carried out, revealing the independence and interconnection of principal and subordinate hierarchy among inter-basin reservoir group in deep level; and applying particle swarm optimization based on immune evolutionary to have hierarchical optimization solution for model. Taking the lower reaches of the Luanhe River inter-basin reservoir group as research object, the calculation results show: ① reduce water loss and increase the utilization ratio of water resource; ② increase the guaranteed rate of water supply in Tangshan and Tianjin as well as lower lack of water damage depth.