Abstract: Reservoir impoundment has changed the temporal and spatial distribution of water temperature and caused ecological problems, such as ecosystem disorder and low-temperature underwater discharge in reservoir areas.This resulted in adverse effects on the growth and reproduction of indigenous fish.The 3-D water temperature model was established using the Delft3D model.The dynamic change process of water temperature in the transverse, longitudinal, and vertical dimensions of the Nuozhadu Reservoir under typical meteorological and hydrological conditions was simulated.Considering this context, and combined with the ecological water temperature demand of downstream fish, a layered water intake scheme of stacked beam doors with ecological water temperature as the goal was formulated.The most suitable location and time for releasing indigenous fish (Tor sinensis) in Nuozhadu Reservoir were determined by combining the requirements of the species involving breeding and release.It was shown in the results that there are differences in the layered water intake scheme of stop logs in different years and the suitable location and time for proliferation and release.The universal layered water intake rules for stop logs were formulated based on the water temperature demand in typical years and the engineering conditions of stop logs.The most suitable location for placing Chinese snakehead fry was the Xiaoheijiang tributary, preferably from August to September.A scientific basis for developing a reservoir ecological dispatching strategy to alleviate the water temperature imbalance may be provided by the results of this study.